DETERMINATION OF TOTAL CYSTEAMINE IN HUMAN SERUM BY A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

Authors
STACHOWICZ M LEHMANN B TIBI A PROGNON P DAURAT V PRADEAU D
Citation
M. Stachowicz et al., DETERMINATION OF TOTAL CYSTEAMINE IN HUMAN SERUM BY A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION, Journal of pharmaceutical and biomedical analysis, 17(4-5), 1998, pp. 767-773
Citations number
18
Categorie Soggetti
Pharmacology & Pharmacy","Chemistry Analytical
Journal title
Journal of pharmaceutical and biomedical analysis → ACNP
ISSN journal
07317085
Volume
17
Issue
4-5
Year of publication
1998
Pages
767 - 773
Database
ISI
SICI code
0731-7085(1998)17:4-5<767:DOTCIH>2.0.ZU;2-U
Abstract
A convenient, reliable and rapid method for the determination of total cysteamine in human plasma by high-performance liquid chromatography with fluorescence detection is reported. This assay involves reduction of samples with dithiothreitol, derivatization of total cysteamine by addition of monobromobimane and protein precipitation by perchloric a cid. The calibration curve was linear in the range 2-150 nmol ml(-1) a nd the detection limit was 0.5 nmol ml(-1). This method was successful ly applied for a pharmacokinetic study of three cysteamine derivatives in healthy volunteers without any interference from coexisting substa nces. (C) 1998 Elsevier Science B.V. All rights reserved.