QUANTITATIVE-DETERMINATION OF GLUCOSE IN BLOOD-PLASMA BY HOMONUCLEAR PROTON DECOUPLED WATER ATTENUATION TRANSVERSE RELAXATION CARR-PURCELL-MEIBOOM-GILL (WATR-HDCPMG) NMR-SPECTROSCOPY

Glucose in 5% D2O/95% H2O solution was successfully determined quantit atively by measurements of the H-1 NMR peak height (intensity) of the single peaks at delta(H-1) = 5.22 and 4.64 ppm corresponding to the al pha-D and the P-D-glucose spectrum, respectively. The single peaks wer e obtained from decoupling of the high field part of the AX spectrum o f the alpha-D- or the P-D-glucose by incorporation of time shared homo nuclear decoupling in the WATR-CPMG method (WATR-HDCPMG) without re-at tenuation of the water peak. The method was applied to the determinati on of total glucose in blood plasma from human subjects undergoing ora l glucose tolerance test (OGTT) in the teaching hospital. The results compared favorably with those obtained from the standard glucose oxida se method obtained in a hospital pathology laboratory. The accuracy of the results obtained using the WATR-HDCPMG method was within 3.5% of the glucose oxidation method.