DETERMINATION OF CR(III) IN URINE, BLOOD-SERUM AND HAIR USING FLOW-INJECTION CHEMILUMINESCENCE ANALYSIS

A flow injection (FI) chemiluminescence method for the determination o f Cr(III) in blood serum, urine and hair samples is reported. It is ba sed on the chromium-catalyzed light emission from the luminol oxidatio n by hydrogen peroxide. The apparatus consists of an FI system with a flow cell formed by a coiled transparent tube suitable for chemilumine scence detection. The specificity of the method is achieved in presenc e of EDTA. The detection limit under optimum conditions is 0.01 mu g L -1 of Cr(III). Precision and accuracy were evaluated by determining Cr (III) concentrations in urine standards from the National Institute of Standard and Technology (NIST).