INFLUENCE OF THE KIND OF ALCOHOLIC MODIFIER ON CHIRAL SEPARATION ON APIRKLE MODEL CHIRAL COLUMN

Separation of the enantiomers of two series of seven organic phosphona tes on pi acidic N-(3,5-dinitrobenoyl) leucine chiral stationary phase is described. Enantiomer resolution is highly dependent on the mobile phase composition and the structure of the enantiomers. One of seven enantiomers can be separated using hexane-2-propanol mobile phase, oth er enantiomers can be partly separated only. The six out of seven enan tiomers are separated and the retention times of all enantiomers are s ignificantly decreased using ethanol instead of 2-propanol. The separa tion of all enantiomers is hardly affected by varying column temperatu re (10-40 degrees C).