RAT-LIVER GENESINE - A BIOCHEMICAL APPROACH

Genesine, the naked autoglucosylating protein involved in glycogen bio synthesis, was partially purified from rat liver and some of its bioch emical properties were characterized. Its activity was strongly activa ted by Mn2+ and two-pH optimums were obtained. UDP-C-14-Glc was the pr eferred glucosyl donor substrate, but also UDP-C-14-Xy1 was. It was ad ditionally found that more than one glucose was transferred to the pro tein or to that alpha 1,4 glucan already linked to the protein from UD P-Glc. Glucose, maltose, xylose and UDP were inhibitors of genesine ac tivity.