SELECTIVE INACTIVATION OF ELONGATION FACTOR-2 (EF-2) IN FREE ADIPOCYTES OBTAINED BY TREATING SARCOPHAGA LARVAL FAT-BODIES WITH CHYMOTRYPSIN

Authors
OKUYAMA H KURATA S HOMMA K NATORI S
Citation
H. Okuyama et al., SELECTIVE INACTIVATION OF ELONGATION FACTOR-2 (EF-2) IN FREE ADIPOCYTES OBTAINED BY TREATING SARCOPHAGA LARVAL FAT-BODIES WITH CHYMOTRYPSIN, Insect biochemistry and molecular biology, 28(5-6), 1998, pp. 301-307
Citations number
20
Categorie Soggetti
Entomology,Biology
Journal title
Insect biochemistry and molecular biology → ACNP
ISSN journal
09651748
Volume
28
Issue
5-6
Year of publication
1998
Pages
301 - 307
Database
ISI
SICI code
0965-1748(1998)28:5-6<301:SIOEF(>2.0.ZU;2-#
Abstract
The molecular mechanism(s) involved in the selective cessation of prot ein synthesis in free adipocytes obtained by treating fat bodies of Sa rcophaga larvae with chymotrypsin were investigated (Okuyama, H., Kura ta, S., Natori, S., 1994. Insect Biochem Mol. Biol. 24, 525-530). Ribo somes prepared from these adipocytes were normal. but the activity of soluble factor(s) needed for protein synthesis was greatly reduced com pared with the control fat bodies. From experiments using diphtheria t oxin, we concluded that EF-2, but not other factors: was selectively i nactivated in these free adipocytes. (C) 1998 Elsevier Science Ltd. Al l rights reserved.