ATOMIC-RESOLUTION STRUCTURE OF HUMAN HBP CAP37/AZUROCIDIN/

Crystals of human heparin binding protein (HBP) diffract to 1.1 Angstr om when flash-frozen at 120 K. The atomic resolution structure has bee n refined anisotropically using SHELXL96. The final model of HBP consi sts of 221 amino-acid residues of 225 possible, three glycosylation un its, one chloride ion, 15 precipitant ethanol molecules and 323 water molecules. The structure is refined to a final crystallographic R fact or of 15.9% and R-free(5%) of 18.9% using all data. A putative protein kinase C activation site has been identified, involving residues 113- 120. The structure is compared to the previously determined 2.3 Angstr om resolution structure of HBP.