CRYSTALLIZATION OF HUMAN-COMPLEMENT COMPONENT C5

Citation
Rg. Discipio et al., CRYSTALLIZATION OF HUMAN-COMPLEMENT COMPONENT C5, Acta crystallographica. Section D, Biological crystallography, 54, 1998, pp. 643-646
Citations number
21
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biophysics,Biology
ISSN journal
09074449
Volume
54
Year of publication
1998
Part
4
Pages
643 - 646
Database
ISI
SICI code
0907-4449(1998)54:<643:COHCC>2.0.ZU;2-2
Abstract
Human complement component C5 has been crystallized using a low-salt b atch technique. The crystals are large hexagonal bipyramids often larg er than 1.5 mm. Although these crystals were grown in low salt (0.1 M NaCl), they are remarkably stable for at least 2 months at 281 K and t hey are not dissolved in aqueous buffers containing up to 2 M sodium c hloride. The space group is P3(1)21 or P3(2)21, and the cell parameter s were determined to be a = 144.9, b = 144.9, c = 243.1 Angstrom; alph a = 90 degrees, beta = 90, gamma = 120 degrees. At room temperature an d cryo-temperatures the crystals diffract at best to 6 Angstrom using rotating-anode X-ray sources. Using synchrotron radiation with cryopro tection using 40%(nu/nu)(-) PEG 400 the resolution limit can be extend ed to 3.3 Angstrom. In both cases the crystals show significant anisot ropy, with relatively weaker reflections at higher resolution in the a b* plane.