CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF PSEUDOMONAS-PUTIDA HISTIDINE AMMONIUM-LYASE

Authors
TEO B KIDD RD MACK J TIWARI A HERNANDEZ D PHILLIPS AT FARBER GK
Citation
B. Teo et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF PSEUDOMONAS-PUTIDA HISTIDINE AMMONIUM-LYASE, Acta crystallographica. Section D, Biological crystallography, 54, 1998, pp. 681-683
Citations number
25
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biophysics,Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
54
Year of publication
1998
Part
4
Pages
681 - 683
Database
ISI
SICI code
0907-4449(1998)54:<681:CAPSOP>2.0.ZU;2-R
Abstract
Histidine ammonium-lyase from P. putida was expressed in Escherichia c oli, purified to homogeneity, and crystallized by the vapour-diffusion method using polyethylene glycol 3350 as the precipitant. The crystal s, which diffract to at least 2.5 Angstrom resolution, exhibit the sym metry of space group P2(1)2(1)2(1), with unit-cell parameters a = 89.7 , b = 138.2 and c = 164.8 Angstrom. The asymmetric unit contains a tet ramer, and the crystals have a V-m value of 2.41 Angstrom(3) Da(-1).