Many feed ingredients in use in monogastric diets contain significant
quantities of antinutritional factors (ANF) which limit both their fee
d value and their use. Almost all enzymes currently being used address
such factors to varying degrees, allowing for more economic utilizati
on of raw materials. However, animal response to xylanase, beta-glucan
ase and even phytase utilization reported in the literature tends to v
ary. Factors such as enzyme source, ingredient variety and environment
under which the ingredient was grown, stored and processed into anima
l feed, age of animal, interaction with other dietary ingredients, and
health status are shown to affect significantly the response obtained
. As a result, the mode of action of xylanases and beta-glucanases is
still debated due to too much emphasis being placed on interpretation
of individual trial results without regard to the interactive factors
or the literature dataset as a whole. Better understanding of such fac
tors will improve data interpretation. While results with phytase are
not subject to such extreme variation, they are nevertheless inconsist
ent in the degree to which inorganic phosphorus can be replaced by thi
s enzyme. Greater understanding of the ANF and factors which interact
to govern the response to added exogenous enzymes will undoubtedly imp
rove the economic return and confidence in their use. Improved knowled
ge of ANF structure will result in development of enzymes directed tow
ards far more specific targets, which enhances the likelihood of succe
ss and should reduce the overall enzyme usage.