HIGHLY ENANTIOSELECTIVE HPLC SEPARATIONS USING THE COVALENTLY BONDED MACROCYCLIC ANTIBIOTIC, RISTOCETIN-A, CHIRAL STATIONARY-PHASE

Authors
EKBORGOTT KH LIU YB ARMSTRONG DW
Citation
Kh. Ekborgott et al., HIGHLY ENANTIOSELECTIVE HPLC SEPARATIONS USING THE COVALENTLY BONDED MACROCYCLIC ANTIBIOTIC, RISTOCETIN-A, CHIRAL STATIONARY-PHASE, Chirality, 10(5), 1998, pp. 434-483
Citations number
32
Categorie Soggetti
Chemistry Medicinal","Chemistry Analytical","Chemistry Inorganic & Nuclear","Pharmacology & Pharmacy
Journal title
Chirality → ACNP
ISSN journal
08990042
Volume
10
Issue
5
Year of publication
1998
Pages
434 - 483
Database
ISI
SICI code
0899-0042(1998)10:5<434:HEHSUT>2.0.ZU;2-Z
Abstract
The macrocyclic glycopeptide, ristocetin A, was covalently bonded to a silica gel support and evaluated as a liquid chromatographic (LC) chi ral stationary phase (CSP). Over 230 racemates were resolved in either the reversed-phase mode, the normal-phase mode, or the polar-organic mode. The retention behavior and selectivity of this CSP were examined in each mode. Optimization of separations on this column is discussed . The ristocetin A CSP appeared to be complimentary to other glycopept ide CSPs (i.e., vancomycin and teicoplanin). Column stability was exce llent. The CSP was not irreversibly altered when going from one mobile phase mode to another. (C) 1998 Wiley-Liss, Inc.