ELECTROSPRAY MASS-SPECTROMETRY FOR ASSAY OF ERYTHROMYCIN-A EXTRACTED FROM FERMENTATION LIQUOR

Authors
MORGAN J ROWLANDS RT JOYCEMENEKSCE ME GILBERT IH LLOYD D
Citation
J. Morgan et al., ELECTROSPRAY MASS-SPECTROMETRY FOR ASSAY OF ERYTHROMYCIN-A EXTRACTED FROM FERMENTATION LIQUOR, Biotechnology techniques, 12(6), 1998, pp. 435-438
Citations number
8
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
6
Year of publication
1998
Pages
435 - 438
Database
ISI
SICI code
0951-208X(1998)12:6<435:EMFAOE>2.0.ZU;2-J
Abstract
Electrospray Mass Spectrometry (ESI-MS) can accurately distinguish and quantify erythromycins by their mass:charge ratios. This procedure is suitable for assay in complex fermentation media, and is compared wit h previous techniques which depend for detection of erythromycin by th e low UV absorbance of erythromycin, after separation of the 5 analogu es by HPLC, TLC, GLC, and with biological assays which employ erythrom ycin-sensitive bacterial strains e.g. of Arthrobacter citreus.