METHODS FOR BACULOVIRUS AMPLIFICATION - APPLICATION TO LARGE-SCALE RECOMBINANT PROTEIN-PRODUCTION IN INSECT CELLS

Authors
ROSS OH MCCABE DD HOLLIS GF ROSENFELD SA
Citation
Oh. Ross et al., METHODS FOR BACULOVIRUS AMPLIFICATION - APPLICATION TO LARGE-SCALE RECOMBINANT PROTEIN-PRODUCTION IN INSECT CELLS, Biotechnology techniques, 12(6), 1998, pp. 463-465
Citations number
6
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
6
Year of publication
1998
Pages
463 - 465
Database
ISI
SICI code
0951-208X(1998)12:6<463:MFBA-A>2.0.ZU;2-T
Abstract
Baculovirus amplification in one insect cell line Spodoptera frugiperd a (Sf21) and subsequent recombinant protein production in another cell line, Trichoplusia ni, has been achieved within a single bioreactor. The advantages of this single bioreactor configuration include minimiz ation of the virus volumes and titres required for large scale protein expression experiments as well as optimization of the infection proce ss itself.