MONITORING OF RIBOSOMES AND SUBUNITS IN ESCHERICHIA-COLI DURING PRODUCTION OF GLUCOSE-ISOMERASE USING FLOW FIELD-FLOW FRACTIONATION

Authors
NILSSON M WAHLUND KG BULOW L
Citation
M. Nilsson et al., MONITORING OF RIBOSOMES AND SUBUNITS IN ESCHERICHIA-COLI DURING PRODUCTION OF GLUCOSE-ISOMERASE USING FLOW FIELD-FLOW FRACTIONATION, Biotechnology techniques, 12(6), 1998, pp. 477-480
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
6
Year of publication
1998
Pages
477 - 480
Database
ISI
SICI code
0951-208X(1998)12:6<477:MORASI>2.0.ZU;2-0
Abstract
Ribosomal components and tRNA of E. coli TG1 producing a recombinant e nzyme, glucose isomerase, were monitored by asymmetrical flow field-fl ow fractionation. The growth temperatures were 20, 30 and 37 degrees C . Results indicate a correlation between translational capacity and th e amount obtained of the recombinant enzyme. Highest yield was obtaine d when cultivating at 30 degrees C.