COMPARISON OF THE EFFICIENCY OF MOLONEY MURINE LEUKEMIA-VIRUS (M-MULV) REVERSE-TRANSCRIPTASE, RNASE H--M-MULV REVERSE-TRANSCRIPTASE AND AVIAN MYELOBLASTOMA LEUKEMIA-VIRUS (AMV) REVERSE-TRANSCRIPTASE FOR THE AMPLIFICATION OF HUMAN-IMMUNOGLOBULIN GENES

Authors
WONG L PEARSON H FLETCHER A MARQUIS CP MAHLER S
Citation
L. Wong et al., COMPARISON OF THE EFFICIENCY OF MOLONEY MURINE LEUKEMIA-VIRUS (M-MULV) REVERSE-TRANSCRIPTASE, RNASE H--M-MULV REVERSE-TRANSCRIPTASE AND AVIAN MYELOBLASTOMA LEUKEMIA-VIRUS (AMV) REVERSE-TRANSCRIPTASE FOR THE AMPLIFICATION OF HUMAN-IMMUNOGLOBULIN GENES, Biotechnology techniques, 12(6), 1998, pp. 485-489
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
6
Year of publication
1998
Pages
485 - 489
Database
ISI
SICI code
0951-208X(1998)12:6<485:COTEOM>2.0.ZU;2-O
Abstract
The efficiency of three commonly used reverse transcriptases, namely M oloney Murine Leukaemia Virus (M-MuLV) reverse transcriptase, RNase H- -M-MuLV reverse transcriptase and Avian Myeloblastoma Leukaemia Virus (AMV) reverse transcriptase (RT), have been compared in RT-PCR amplifi cation of antibody genes. These enzymes differed significantly in thei r efficiency of cDNA synthesis as indicated by the degree of V gene am plification by PCR; Moloney Murine Leukaemia Virus (M-MuLV) reverse tr anscriptase and RNase H--M-MuLV reverse transcriptase produced greater amplification of the human V-gene repertoire using RT-PCR than that o f the Avian Myeloblastoma Leukaemia Virus (AMV) reverse transcriptase.