CALCULATIONS ON O-2 TRANSFER IN CAPSULES WITH ANIMAL-CELLS FOR THE DETERMINATION OF MAXIMUM CAPSULE SIZE WITHOUT O-2 LIMITATION

Authors
KIM SK YU SH SON JH HUBNER H BUCHHOLZ R
Citation
Sk. Kim et al., CALCULATIONS ON O-2 TRANSFER IN CAPSULES WITH ANIMAL-CELLS FOR THE DETERMINATION OF MAXIMUM CAPSULE SIZE WITHOUT O-2 LIMITATION, Biotechnology letters, 20(6), 1998, pp. 549-552
Citations number
5
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
20
Issue
6
Year of publication
1998
Pages
549 - 552
Database
ISI
SICI code
0141-5492(1998)20:6<549:COOTIC>2.0.ZU;2-T
Abstract
The size of the capsules without O-2 limitation and maximum allowable capsule size for different cell densities were calculated by the obser vable modulus (modified Thiele modulus). When hybridoma (ATCC HB-8852) at 2.5 x 10(5)cells/ml was encapsulated and grown, the cell density r eached to 2.6 x 10(7)cells/ml in the 7th day of incubation. The averag e diameter of the capsule was 2.1mm. The maximum cell density obtained from experiment agreed with the calculated cell density for the given size of the capsules.