BFIL, A RESTRICTION-ENDONUCLEASE FROM BACILLUS-FIRMUS S8120, WHICH RECOGNIZES THE NOVEL NON-PALINDROMIC SEQUENCE 5'-ACTGGG(N)(5 4)-3'/

Authors
VITKUTE J MANELIENE Z PETRUSYTE M JANULAITIS A
Citation
J. Vitkute et al., BFIL, A RESTRICTION-ENDONUCLEASE FROM BACILLUS-FIRMUS S8120, WHICH RECOGNIZES THE NOVEL NON-PALINDROMIC SEQUENCE 5'-ACTGGG(N)(5 4)-3'/, Nucleic acids research, 26(14), 1998, pp. 3348-3349
Citations number
7
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
26
Issue
14
Year of publication
1998
Pages
3348 - 3349
Database
ISI
SICI code
0305-1048(1998)26:14<3348:BARFBS>2.0.ZU;2-N
Abstract
A new type IIS restriction endonuclease Bfil has been partially purifi ed from Bacillus firmus S8120. Bfil recognizes the non-palindromic hex anucleotide sequence 5'-ACTGGG(N)(5/4)-3' and makes a staggered cut at the fifth base pair downstream of the recognition sequence on the upp er strand, producing a single base 3' protruding end.