EFFICIENT MODIFICATION OF A HUMAN-CHROMOSOME BY TELOMERE-DIRECTED TRUNCATION IN HIGH HOMOLOGOUS RECOMBINATION-PROFICIENT CHICKEN DT40 CELLS

Authors
KUROIWA Y SHINOHARA T NOTSU T TOMIZUKA K YOSHIDA H TAKEDA S OSHIMURA M ISHIDA I
Citation
Y. Kuroiwa et al., EFFICIENT MODIFICATION OF A HUMAN-CHROMOSOME BY TELOMERE-DIRECTED TRUNCATION IN HIGH HOMOLOGOUS RECOMBINATION-PROFICIENT CHICKEN DT40 CELLS, Nucleic acids research, 26(14), 1998, pp. 3447-3448
Citations number
8
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
26
Issue
14
Year of publication
1998
Pages
3447 - 3448
Database
ISI
SICI code
0305-1048(1998)26:14<3447:EMOAHB>2.0.ZU;2-A
Abstract
Truncation of human chromosomes at desired sites by homologous recombi nation techniques enables functional and structural analyses of human chromosomes and development of human artificial chromosomes. However, this targeted truncation has been inefficient. We describe here an eff icient method for targeted truncation in the chicken DT40 cells with a high homologous recombination rate. The human chromosome 22 was trans ferred into DT40 cells, where human telomeric repeat (TTAGGG)n was tar geted to the LIF locus on the chromosome. Molecular and cytogenetic an alyses showed that the predicted truncation at the LIF locus occurred in all of the targeted clones.