The envelope protein HIV-1 gp41 has been shown to exert various effect
s on human T-cells, B-cells and monocytes like inhibition of cell prol
iferation, modulation of MHC expression and cytokine production. In co
ntrast to gp120, where several receptor molecules have been identified
, the receptor for gp41 is still unknown. Using a sepharose column, co
upled with recombinant soluble gp41, (rsgp41; Env amino acids 539-684)
, five gp41-binding proteins of 37, 45, 50, 62 and 100 kDa had been is
olated from lysates of the B-cell line Raji. Two mouse antiserums were
generated against the proteins P45 and P62 and were tested against th
e binding specificity of both antiserums. In Western blot analysis the
antiserums recognized two protein bands of 45 and 62 kDa in complete
Raji cell lysates, as well as the purified proteins P45 and P62, respe
ctively, but did not show any cross-reaction, indicating that the two
proteins do not share any immunological epitopes. Besides, the polyclo
nal antiserums did not recognize the other gp41-binding proteins P37,
P50 and P100. Using the P62 antiserum proteins of the same size as in
Raji cell lysates were stained in the lysates of the monocytic cell li
ne U937 and the T-cell line H9, demonstrating distribution of P62 in d
ifferent blood cells. P45 seems not to be identical to HLA-C which had
been shown to bind to gp41. These results indicate, that P45 and P62
are two separate gp41-binding proteins without homology to each other
or to the other gp41-binding proteins. Published by Elsevier Science B
.V. All rights reserved.