A HIGH-RESOLUTION CYTOGENETIC MAP OF HUMAN CHROMOSOME-12 - LOCALIZATION OF 195 NEW COSMID MARKERS BY DIRECT R-BANDING FLUORESCENCE IN-SITU HYBRIDIZATION

We have constructed a high-resolution cytogenetic map of human chromos ome 12 with 195 newly isolated cosmids by direct R-banding flourescenc e in situ hybridization. The fluorescent signals of 195 clones were ev enly distributed throughout chromosome 12, but sublocalized preferenti ally to R-positive bands. This high-resolution cytogenetic.map with an average map distance of 0.73 Mb on bands can, in conjunction with a g enetic linkage map, facilitate the analysis of chromosomal and molecul ar aberrations in genetic diseases and cancers. Moreover, the cytogene tic mapping data provide starting points for establishing contig maps with cosmid clones and yeast artificial chromosomes.