ENGINEERING MOLECULAR RECOGNITION IN ALKANE OXIDATION CATALYZED BY CYTOCHROME P450(CAM)

Authors
STEVENSON JA BEARPARK JK WONG LL
Citation
Ja. Stevenson et al., ENGINEERING MOLECULAR RECOGNITION IN ALKANE OXIDATION CATALYZED BY CYTOCHROME P450(CAM), New journal of chemistry, 22(6), 1998, pp. 551-552
Citations number
12
Categorie Soggetti
Chemistry
Journal title
New journal of chemistry → ACNP
ISSN journal
11440546
Volume
22
Issue
6
Year of publication
1998
Pages
551 - 552
Database
ISI
SICI code
1144-0546(1998)22:6<551:EMRIAO>2.0.ZU;2-G
Abstract
The activity, selectivity and coupling efficiency of alkane oxidation by the monooxygenase enzyme cytochrome P450(cam) can be enhanced by en gineering the active site topology to accommodate different alkanes: r educing the active site volume by the Y96F-V247L double mutations resu lted in four-fold higher activity for the oxidation of hexane (1) than 3-methylpentane (2) while the larger active site of the Y96A-V247A do uble mutant gave rise to a two-fold preference for 2 over 1.