ANTIPROLIFERATIVE GENE BTG1 IS HIGHLY EXPRESSED IN APOPTOTIC CELLS INMACROPHAGE-RICH AREAS OF ADVANCED LESIONS IN WATANABE HERITABLE HYPERLIPIDEMIC RABBIT AND HUMAN
Mh. Corjay et al., ANTIPROLIFERATIVE GENE BTG1 IS HIGHLY EXPRESSED IN APOPTOTIC CELLS INMACROPHAGE-RICH AREAS OF ADVANCED LESIONS IN WATANABE HERITABLE HYPERLIPIDEMIC RABBIT AND HUMAN, Laboratory investigation, 78(7), 1998, pp. 847-858
Citations number
31
Categorie Soggetti
Pathology,"Medical Laboratory Technology","Medicine, Research & Experimental
In the present study, the expression and potential role of the highly
conserved antiproliferative gene BTG1 in the process of apoptosis as i
t occurs in atherosclerotic lesions was examined. In situ hybridizatio
n and immunodetection studies demonstrated that BTG1 localized to spec
ific macrophage-rich regions of lesions in both Watanabe heritable hyp
erlipidemic rabbits and humans. In addition, the spatial distribution
of BTG1 mRNA was shown to colocalize not only with macrophage-rich reg
ions but also to cells that exhibited changes consistent with apoptosi
s, such as DNA fragmentation and nuclear condensation. In vitro studie
s demonstrated that forced overexpression of BTG1 induced a S-fold inc
rease in apoptosis in NIH/3T3 cells. Furthermore, significantly increa
sed expression of BTG1 mRNA resulted from lipid loading of human monoc
yte-derived macrophages in vitro. The process of increasing lipid cont
ent in macrophages is frequently associated with decreased macrophage
viability. Taken together, these data underscore a potentially importa
nt role for BTG1 in regulating the cellularity of advanced atheroscler
otic lesions.