N. Sakairi et al., A METHOD FOR DIRECT HARVEST OF BACTERIAL CELLULOSE FILAMENTS DURING CONTINUOUS CULTIVATION OF ACETOBACTER-XYLINUM, Carbohydrate polymers, 35(3-4), 1998, pp. 233-237
Continuous filamentation of bacterial cellulose (BC) was successfully
achieved by using shallow pan for the incubation to regulate thickness
of the BC gel produced by Acetobacter xylinum. The BC filament was ha
rvested and prepared directly by picking up BC pellicles, the thin BC
gel, and winding slowly from the surface of the culture medium passed
through a preliminary bactericidal washing bath. The X-ray diffraction
analysis and scanning electron microscopic observation of the BC fila
ment thus obtained showed that the filament was smooth and the fairly
good orientation of BC molecules. The average tensile strength was 4.4
g denier(-1) for the filament prepared by hot alkaline treatment and
subsequent washing with distilled water and dried under tension (Filam
ent W): 3.4 g denier(-1) for washing with 10% aqueous ethylene glycol
after alkaline treatment followed by drying under tension (Filament E)
and 2.4 g denier(-1) for the treatment with 10% ethylene glycol after
normal water-washing followed by drying under tension. (C) 1998 Elsev
ier Science Ltd. All rights reserved.