EVALUATION OF AVIDIN-BIOTIN BINDING USING A BIOTIN-LABELED WITH DAUNOMYCIN BY PHOTOACOUSTIC SPECTROMETRY

Authors
SUGAWARA K YAMAGUCHI Y NAGO M FUTAMATA M HOSHI S AKATSUKA K
Citation
K. Sugawara et al., EVALUATION OF AVIDIN-BIOTIN BINDING USING A BIOTIN-LABELED WITH DAUNOMYCIN BY PHOTOACOUSTIC SPECTROMETRY, Chemistry Letters, (7), 1998, pp. 627-628
Citations number
9
Categorie Soggetti
Chemistry
Journal title
Chemistry Letters → ACNP
ISSN journal
03667022
Issue
7
Year of publication
1998
Pages
627 - 628
Database
ISI
SICI code
0366-7022(1998):7<627:EOABUA>2.0.ZU;2-N
Abstract
A method of evaluation for avidin-biotin binding using photoacoustic s pectrometry (PAS) was developed. Biotin labeled with daunomycin was pr epared. The avidin-biotin binding between avidin and the labeled bioti n (LB) makes avidin colored. This is because the part of daunomycin is taken in biotin sites of avidin. Therefore, the concentration of avid in could be estimated based on measurement of the LB with avidin by PA S.