This paper is concerned with the microbiological uptake of heavy metal
s in slow sand filtration (SSF) and the determination of active biomas
s by organic nitrogen analysis described herein. The heavy metal accum
ulation and the total biomass in the schmutzdecke scrapings were deter
mined and compared. The heavy metals were extracted from the sand scra
pings by means of HNO3-HCl digestion. The percentage nitrogen content
of volatile solids (VS) for the same sand scrapings were determined, a
nd this measure was taken as being directly equivalent to the biomass
in the schmutzdecke scrapings. The results show that the more the biom
ass, the greater the metal accumulation, which indicates microbiologic
al accumulation/uptake rather than simple adsorption of heavy metals i
n SSF.