EFFECTS OF CYCLOSPORINE-A AND IBUPROFEN ON LIVER ISCHEMIA-REPERFUSIONINJURY IN THE RAT

Liver ischemia followed by reperfusion is an important and common clin ical event associated with the activation of an endogenous inflammator y response. The aim of this study was to determine the effects of cycl osporin A (CsA) and ibuprofen (IBU) on ischemia-reperfusion injury. Ma le Spraque-Dawley rats were subjected to 60 min of total liver ischemi a followed by 120 min reperfusion. Liver tissue samples were obtained for measurements of malondialdehyde (MDA) levels as an index of tissue lipid peroxidation by thiobarbituric acid assay. Three groups of anim als were pre-treated with CsA or IBU or both. Treatment with these age nts was given at onset reperfusion after ischemia. The other groups we re designated as ischemic controls, non-ischemic controls and reperfus ion group without treatment. Ischemic control animals showed increased liver MDA levels and in the reperfusion group MDA levels were signifi cantly higher than ischemic levels. CsA and IBU-treated animals had be tter survival and diminished liver MDA levels. The most significant de crease MDA levels was observed in the group treated with two agents wh ich were given together. Serum enzyme levels were significantly higher in the reperfusion group than in the ischemic controls and the enzyme levels were significantly diminished after the treatments. This study suggests that CsA and IBU may be important agents in modulating lipid peroxidation in ischemia-reperfusion liver injury, and combined thera py with these agents may be more effective in treatment of ischemia-re perfusion injury, (C) 1998 Elsevier Science B.V. All rights reserved.