T. Jamsa et al., COMPARISON OF 3-POINT BENDING TEST AND PERIPHERAL QUANTITATIVE COMPUTED-TOMOGRAPHY ANALYSIS IN THE EVALUATION OF THE STRENGTH OF MOUSE FEMUR AND TIBIA, Bone (New York, N.Y.), 23(2), 1998, pp. 155-161
We compared the mechanical and densitometric testing methods in evalua
ting the mechanical strength of mouse cortical hones, The femora and t
ibiae of 10 male mice (weight 32.8 +/- 4.0 g) mere utilized, Volumetri
c cortical bone mineral density (VCtBMD), cross-sectional cortical are
a at midshaft (CSA), cross-sectional moment of inertia (CSMI), and str
ength strain index (SSI) mere measured by peripheral quantitative comp
uted tomography (pQCT), The precision of pQCT expressed as a coefficie
nt of variation (CV) was 1.1%, 2.7%, and 6.4% for vCtBMD, CSA, and CSM
I, respectively. The mechanical properties were measured by a three-po
int bending test. The method error measured from paired bones mas 7.3%
-10.1% for breaking bending force, 15.0%-15.2% for stiffness, 2.0%-2.4
% for vCtBMD, 5.2%-6.4% for CSA, 13.5%-17.6% for CSMI, and 8.9%-18.1%
for SSI, CSMI and CSA mere found to be the best explanatory variables
for the breaking force of femur and tibial respectively, while CSI-I a
nd CSMI were the best predictors for the elastic modulus of femur and
tibia, respectively, CSA had a higher correlation with mechanical para
meters than VCtBMD. On the basis of this study, the mechanical tests a
nd the pQCT measurements are relevant in biomechanical studies on mous
e bones and justify the use of the murine model, High-resolution pQCT
gives better precision than the three-point bending test in studies of
mouse bones. (Bone 23:155-161; 1998) (C) 1998 by Elsevier Science Int
. All rights reserved.