CH DOMAINS REVISITED

A sequence motif of about 100 amino acids, termed the 'calponin homolo gy domain' has been suggested to confer actin binding to a variety of cytoskeletal and signalling molecules, Here we analyse and compare the sequences of all calponin homology domain-containing proteins identif ied to date. We propose that single calponin homology domains do not c onfer actin-binding per se and that the actin-binding motifs of crossl inking proteins, which comprise two disparate calponin homology domain s, represent a unique protein module, (C) 1998 Federation of European Biochemical Societies.