ISOLATION AND CHARACTERIZATION OF UDP-GLUCOSE - CYANIDIN 3-O-GLUCOSYLTRANSFERASE FROM THE FLOWER BUDS OF SENECIO X HYBRIDUS

Authors
OGATA J TERAMOTO S YOSHITAMA K
Citation
J. Ogata et al., ISOLATION AND CHARACTERIZATION OF UDP-GLUCOSE - CYANIDIN 3-O-GLUCOSYLTRANSFERASE FROM THE FLOWER BUDS OF SENECIO X HYBRIDUS, Journal of plant research, 111(1102), 1998, pp. 213-216
Citations number
11
Categorie Soggetti
Plant Sciences
Journal title
Journal of plant research → ACNP
ISSN journal
09189440
Volume
111
Issue
1102
Year of publication
1998
Pages
213 - 216
Database
ISI
SICI code
0918-9440(1998)111:1102<213:IACOU->2.0.ZU;2-N
Abstract
An UDPG: cyanidin 3-O-glucosyltransferase was isolated and purified ab out 260-fold from the flower buds of Senecio X hybridus. The enzyme sh owed a pH optimum of 7.5 and no additional cofactors were required. Th e Km values for cyanidin and UDPG were 0.33 and 0.20 mM, respectively. Rs molecular mass estimated by Sephacryl S-200 chromatography was 52 kDa.