ISOLATION AND NUCLEOTIDE-SEQUENCE OF THE GENE ENCODING PHOSPHOENOLPYRUVATE CARBOXYKINASE FROM KLUYVEROMYCES-LACTIS

The KIPCK1 gene encoding phosphoenolpyruvate carboxykinase (PEPCK; ATP -dependent) was cloned from the Kluyveromyces lactis genome using a PC R amplicon from Saccharomyces cerevisiae PCK1 gene as a probe. A DNA f ragment of about 4.8 kb containing KIPCK1 complemented PEPCK activity of the mutant of S. cerevisiae defective in PEPCK. The KIPCK1 gene has an open reading frame of 1629 bp (543 amino acids). The KIPCK1 nucleo tide sequence and deduced amino acid sequence showed 76% and 84% homol ogies to those of S. cerevisiae PCK1, respectively. Multiple alignment of ATP-dependent PEPCK genes shows highly conserved regions. The nucl eotide sequence of KIPCK1 has been submitted to the DDBJ/GenBank/EMBL data bank with Accession Number U88575. (C) 1998 John Wiley & Sons, Lt d.