GENETIC-ANALYSIS OF GLUFOSINATE RESISTANCE IN CROSSES BETWEEN TRANSFORMED RICE (ORYZA-SATIVA) AND RED RICE (ORYZA-SATIVA)

Reciprocal controlled crosses were made in the greenhouse between Gulf mont rice transformed with the bialaphos resistance (BAR) gene and red rice and BAR-transformed Koshihikari rice and red rice to assess the inheritance of glufosinate resistance. All F-1, plants were resistant to 2.2 kg ai/ha glufosinate, Ammonia accumulation as a measure of gluf osinate resistance in the F-1, hybrids was assayed at 4 and 8 days aft er treatment (DAT), Ammonia accumulation in hybrids 4 DAT was similar to glufosinate treated, transformed rice, while treated nontransformed plants accumulated 14 to 23 times more ammonia compared with the hybr ids. The nature of inheritance of glufosinate resistance in F-2, rice plants was studied by a glufosinate dip test, a spray test, and ammoni a assay. All three tests confirmed that glufosinate resistance, as inf luenced by the BAR gene, segregated in a 3 (resistant): 1 (susceptible ) ratio.