DIFFERENTIAL PRODUCTION OF MONOCLONAL-ANTIBODIES TO CARBOHYDRATE MOIETY OR PEPTIDES MOIETY OF GLYCOPROTEINS BY DIFFERENT ROUTES OF IMMUNIZATION

The administration of ABO blood group active glycoproteins via the rea r footpads of BALB/c mice with subsequent fusion of popliteal lymph no de lymphocytes produced hybridomas differentially secreting antibodies to the peptide moiety of antigens, On the other hand, conventional in traperitoneal immunization of the same antigens and intraperitoneal bo ost followed by splenic lymphocyte fusion produced hybridomas differen tially secreting antibodies to the carbohydrate moiety of antigens, Si milar results were obtained in the production of monoclonal antibodies to erythrocyte membranes. Almost all hybridomas obtained by rear food pad immunization secreted antibodies to the peptide moiety of erythroc yte membrane proteins, and those obtained by intraperitoneal immunizat ion secreted antibodies to both peptide and carbohydrate moieties of e rythrocyte membrane components. These results suggest the possibility of differential production of monoclonal antibodies to the carbohydrat e moiety and the peptide moiety of some glycoproteins by different imm unization routes.