DETERMINATION OF ENZYME-KINETICS AND CHEMOMETRIC EVALUATION OF REACTION-PRODUCTS BY FTIR SPECTROSCOPY ON THE EXAMPLE OF BETA-FRUCTOFURANOSIDASE

Fourier transform infrared (FTIR) spectroscopy in combination with a s equential injection analysis (SIA) manifold was used for the investiga tion of the kinetic properties of the quantitative analysis of the rea ction mixtures. Different sucrose solutions were prepared automaticall y and with the quantitative results provided by the chemometric spectr a evaluation, the Michaelis-Menten constant (K-m) was estimated to be 43 mmol/l. (C) 1998 Elsevier Science B.V. All rights reserved.