G. Hillman et H. Greven, PROTEOGLYCANS (PGS) IN THE LARVAL AMPHIBIAN TOOTH AS VISUALIZED BY CUPROLINIC BLUE, Acta histochemica, 100(3), 1998, pp. 271-278
Proteoglycans (PGs) were localized in the predentine and dentine of yo
ung larvae from the urodelan species Salamandra salamandra. After cupr
olinic blue (CB) staining at the critical electrolyte concentration of
0.1 M MgCl2, CB-positive, electron dense filaments with considerable
variations in length and width were found in the collagen-free zone ad
jacent to the odontoblast processes (length up to 1.3 mu m, width 21 n
m), in predentine (660 nm/ 3.2 nm), in dentine around (20 nm/9 nm) and
in the dentine tubules (35-150 nm/8 nm). Size classes very likely rep
resent different PGs.