ANALYSIS OF MSH3 IN ENDOMETRIAL CANCERS WITH DEFECTIVE-DNA MISMATCH REPAIR

Citation
Em. Swisher et al., ANALYSIS OF MSH3 IN ENDOMETRIAL CANCERS WITH DEFECTIVE-DNA MISMATCH REPAIR, Journal of the Society for Gynecologic Investigation, 5(4), 1998, pp. 210-216
Citations number
40
Categorie Soggetti
Obsetric & Gynecology
ISSN journal
10715576
Volume
5
Issue
4
Year of publication
1998
Pages
210 - 216
Database
ISI
SICI code
1071-5576(1998)5:4<210:AOMIEC>2.0.ZU;2-O
Abstract
OBJECTIVE: To clarify the origin of defective mismatch repair (MMR) in sporadic endometrial cancers with microsatellite instability (MSI), a thorough mutation analysis was performed on the human mismatch repair gene MSH3. METHODS: Twenty-eight MSI-positive endometrial cancers wer e investigated for mutations in the human mismatch repair gene MSH3 us ing single-strand conformation variant (SSCV) analysis of all 24 exons . All variants were sequenced. Loss of heterozygosity was investigated at all MSH3 polymorphisms discovered. A subset of tumors were investi gated for methylation of the 5' promotor region of MSH3 using Southern blot hybridization. RESULTS: An identical single-base deletion (Delta A) predicted to result in a truncated protein was discovered in six t umors (21.4%). This deletion occurs in a string of eight consecutive a denosine residues (A(8)). Because simple repeat sequences are unstable in cells with defective MMR, the observed mutation may be an effect, rather than a cause, of MSI. Evidence of inactivation of the second MS H3 allele in tumours with the Delta A mutation would strongly support a casual role for these MSH3 mutations. However, there was no evidence of a second mutation, loss of sequences, or methylation of the promot er region in any of the tumors with the Delta A mutation. CONCLUSION: Although Delta A mutation is a frequent event in sporadic MSI-positive endometrial cancers, it may not be casually associated with defective DNA MMR. (J Soc Gynecol Invest 1998; 5:210-216). Copyright (C) 1998 b y the Society for Gynecologic Investigation.