DEVELOPMENT OF AN ISOTOPE-SELECTIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY DETECTOR USING CHEMICAL-REACTION-INTERFACE MASS-SPECTROMETRY - APPLICATION TO DEUTERATED CORTISOL METABOLITES IN URINE

Authors
TEFFERA Y ABRAMSON FP MCLEAN M VESTAL M
Citation
Y. Teffera et al., DEVELOPMENT OF AN ISOTOPE-SELECTIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY DETECTOR USING CHEMICAL-REACTION-INTERFACE MASS-SPECTROMETRY - APPLICATION TO DEUTERATED CORTISOL METABOLITES IN URINE, Journal of chromatography. Biomedical applications, 620(1), 1993, pp. 89-96
Citations number
18
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography. Biomedical applications → ACNP
ISSN journal
03784347
Volume
620
Issue
1
Year of publication
1993
Pages
89 - 96
Database
ISI
SICI code
0378-4347(1993)620:1<89:DOAIHL>2.0.ZU;2-Q
Abstract
An isotope-selective detector for HPLC based on the particle-beam-inte rface and the chemical-reaction-interface mass spectrometer (CRIMS) pr inciple is described. This paper focuses on the selective detection of deuterium-labeled analytes. The CRIMS product HD is detected as has b een previously described for GC-CRIMS. The analytical performance was not affected by analyte structure or solvent composition. Deuterium de tection was linear from 20 ng to 490 ng using H-2(3)-labeled cortisol. Based on this method, the fractional abundance of three labeled metab olites of cortisol were determined in the urine of a patient infused w ith tracer amounts of deuterated cortisol.