ELECTRICALLY-INDUCED NEURITE OUTGROWTH OF PC12 CELLS ON THE ELECTRODESURFACE

Citation
K. Kimura et al., ELECTRICALLY-INDUCED NEURITE OUTGROWTH OF PC12 CELLS ON THE ELECTRODESURFACE, Medical & biological engineering & computing, 36(4), 1998, pp. 493-498
Citations number
27
Categorie Soggetti
Engineering, Biomedical","Computer Science Interdisciplinary Applications","Medical Informatics
ISSN journal
01400118
Volume
36
Issue
4
Year of publication
1998
Pages
493 - 498
Database
ISI
SICI code
0140-0118(1998)36:4<493:ENOOPC>2.0.ZU;2-P
Abstract
Morphological differentiation of PC12 cells cultured on an indium-tin oxide (ITO) electrode has been induced to grow neurites in the absence of nerve growth factor (NGF) by electrical stimulation. Rectangular p ulse wave potentials were applied to the electrode at amplitudes of 20 0 mV and 400 mV with frequencies of 50 Hz, 500 Hz, and 1 kHz. The PC12 cells differentiated most prominently at 200 mV with 100 Hz. No stati stically significant differences were observed among the electrically induced neurite lengths. The electrically induced differentiation was completely inhibited by a blockade of calcium influx using LaCl3. This indicates that repeated potential shift in the vicinity of a cellular membrane may stimulate morphological response, probably through calci um ion channels.