Bh. Maraj et al., DETAILED GENETIC-MAPPING AROUND A PUTATIVE PROSTATE-SPECIFIC MEMBRANEANTIGEN LOCUS ON HUMAN-CHROMOSOME 11P11.2, Cytogenetics and cell genetics, 81(1), 1998, pp. 3-9
Physical mapping of the human prostate-specific membrane antigen gene
(FOLH) has not been straightforward. Previously, localisations of this
gene to either 11p11.2 or 11q14 have been described. This raised the
possibility of the presence of more than one related FOLH gene in man.
We now present detailed characterisation of the region around a FOLH
gene in a 500-kb non-chimaeric YAC clone, putatively assigned to 11p11
.2. This clone contains two known microsatellites, D11S1326 and D11S13
57 which have been previously mapped unequivocally to the 11p11.2 regi
on at 62.5 cM. This data strongly supports the original 11p11.2 locali
sation of FOLH. The YAC clone also has a putative EST at each of its e
nds and these have thus been physically positioned on this chromosome.
However, the two regions previously highlighted at 11p11.2 and 11q14
are apparently extensively duplicated, as evidenced by the appearance
of dual FISH signals with a number of different genomic probes selecte
d across this 500-kb 11p11.2 region.