A HIGHLY SELECTIVE AFFINITY LABELING OF PHOSPHOFRUCTOKINASE FROM COTTOCOMEPHORUS-GREWINGKI

Authors
TULOKHONOV II MUSTAEV AA ZAICHIKOV EF
Citation
Ii. Tulokhonov et al., A HIGHLY SELECTIVE AFFINITY LABELING OF PHOSPHOFRUCTOKINASE FROM COTTOCOMEPHORUS-GREWINGKI, Bioorganiceskaa himia, 24(6), 1998, pp. 422-425
Citations number
16
Categorie Soggetti
Chemistry Inorganic & Nuclear",Biology
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
24
Issue
6
Year of publication
1998
Pages
422 - 425
Database
ISI
SICI code
0132-3423(1998)24:6<422:AHSALO>2.0.ZU;2-Q
Abstract
The 80-kDa subunit of phosphofructokinase from the Baikalian fish Cott ocomephorus grewingki was labeled with high selectivity by a two-stage procedure of affinity labeling using p-formylbenzyl fructose 6-phosph ate and [gamma-P-32]ATP. The extent of labeling depended on the presen ce of such effecters as AMP or fructose 1,6-diphosphate in the reactio n mixture.