Ii. Tulokhonov et al., A HIGHLY SELECTIVE AFFINITY LABELING OF PHOSPHOFRUCTOKINASE FROM COTTOCOMEPHORUS-GREWINGKI, Bioorganiceskaa himia, 24(6), 1998, pp. 422-425
The 80-kDa subunit of phosphofructokinase from the Baikalian fish Cott
ocomephorus grewingki was labeled with high selectivity by a two-stage
procedure of affinity labeling using p-formylbenzyl fructose 6-phosph
ate and [gamma-P-32]ATP. The extent of labeling depended on the presen
ce of such effecters as AMP or fructose 1,6-diphosphate in the reactio
n mixture.