EFFECT OF SURFACTANTS ON PEROXIDASE-ACTIVITY - II - EFFECT OF CATIONIC SURFACTANTS

The effect of various cationic surfactants on the initial rate of the 5-aminosalicylic acid peroxidation with horseradish peroxidase was stu died. With increasing concentration of the surfactant, the rate first increased and then decreased. We attributed these changes to a primary activation of the enzyme and/or an increase in the concentration of t he substrate in the region of the active site and to the subsequent in activation of the protein with surfactant followed by its denaturing, respectively.