ANOMER-SELECTIVE GLUCOSYLATION OF L-MENTHOL BY YEAST ALPHA-GLUCOSIDASE

Citation
H. Nakagawa et al., ANOMER-SELECTIVE GLUCOSYLATION OF L-MENTHOL BY YEAST ALPHA-GLUCOSIDASE, Bioscience, biotechnology, and biochemistry, 62(7), 1998, pp. 1332-1336
Citations number
23
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
ISSN journal
09168451
Volume
62
Issue
7
Year of publication
1998
Pages
1332 - 1336
Database
ISI
SICI code
0916-8451(1998)62:7<1332:AGOLBY>2.0.ZU;2-W
Abstract
l-Menthol was glucosylated by the alpha-glucosidase (EC 3.2.1.20) of S accharomyces cerevisiae using maltose as the glucosyl donor. When 50 m g of l-menthol and 1.6 M maltose in 10 mM citrate-phosphate buffer (pH 5.5) were incubated at 45 degrees C, l-menthyl alpha-D-glucopyranosid e (alpha-MenG) was alpha-anomer-selectively formed as a product. The s pecificity of the alpha-linkage was confirmed by C-13-NMR analysis. In the reaction mixture after 2 h, alpha-MenG was mainly accumulated in a crystalline form and the concentration of dissolved alpha-MenG was c onstant at 1.4 mM. The molar conversion yield of alpha-MenG produced b ased on the supplied l-menthol was maximally 30.7% at 48 h of reaction .