TRIACYLGLYCEROL COMPOSITION OF PINUS-KORAIENSIS SEED OIL

The triacylglycerol (TG) composition of Pinus koraiensis seed oil, whi ch contains Delta 5 nonmethylene-interrupted (NMI) fatty acids (FA) (t he main acid is pinolenic, 18.3 Delta 5,9,12), was determined. TG were preliminarily separated by argentation thin-layer chromatography (TLC ), and the obtained fractions were analyzed by high-temperature gas ch romatography (CC) on a capillary column with methyl phenyl silicone ph ase. Additionally, high-performance liquid chromatography (HPLC) of TG was applied. The FA composition of all TC fractions was identified. T he identification of TG was carried out by combining TLC, GC, HPLC, an d calculated equivalent carbon numbers of TG standards. The TC species identification was confirmed by comparison of the theoretical recalcu lated and directly analyzed FA compositions of all TLC fractions of TC . Species of TC with unsaturation degrees of 1 to 7 and trace amounts of saturated and octaenoic TC species were found. Except for minor com pounds, 26 TC molecular species of 32 main components were quantitativ ely determined. The main species were oleoyl dilinoleoylglycerol (14.7 %), dilinoleoyl pinolenoylglycerol (10.7%), palmitoyl oleoyl linoleoyl glycerol (8.3%), triolein (7.6%), and dioleoyl, linoleoylglycerol (7.4 %). Seven TG species contained Delta 5 NMI acyl groups. Of these, the major were dilinoleoyl pinolenoylglycerol (10.7%), stearoyl linoleoyl pinolenoylglycerol (6.5%) dioleoyl, pinolenoylglycerol (5.4%), and pal mitoyl linoleoyl pinolenoyl-glycerol (5.5%). TG species with two or th ree NMI acyl groups were not detected.