DETECTION OF SPECIFIC BACTERIOCIN-PRODUCING LACTIC-ACID BACTERIA BY COLONY HYBRIDIZATION

Authors
MARTINEZ MI RODRIGUEZ E MEDINA M HERNANDEZ PE RODRIGUEZ JM
Citation
Mi. Martinez et al., DETECTION OF SPECIFIC BACTERIOCIN-PRODUCING LACTIC-ACID BACTERIA BY COLONY HYBRIDIZATION, Journal of applied microbiology, 84(6), 1998, pp. 1099-1103
Citations number
18
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Journal of applied microbiology → ACNP
ISSN journal
13645072
Volume
84
Issue
6
Year of publication
1998
Pages
1099 - 1103
Database
ISI
SICI code
1364-5072(1998)84:6<1099:DOSBLB>2.0.ZU;2-Q
Abstract
A colony hybridization method for detecting lactic acid bacteria encod ing specific bacteriocins was developed. Specific PCR-generated probes were used to detect colonies of pediocin PA-1, lactococcin A, enteroc in AS-48, nisin A and lacticin 481 producing strains. The probes were shown to be sensitive and specific for sequences belonging to the stru ctural genes of the respective bacteriocins.