EVIDENCE FOR THE EXISTENCE OF CYTOCHROME B558 IN FISH NEUTROPHILS BY POLYCLONAL ANTIPEPTIDE ANTIBODY

When western blotting analysis of Japanese eel neutrophil lysate was p erformed using antibody against the synthetic peptide corresponding to the carboxyl terminal region of human cytochrome b558 large subunit, a broad band was specifically detected at approximately 90 kDa. The an tibody recognized the epitope present in eel neutrophils only after th e cells were permeabilized with detergent. These results indicate that the large subunit of cytochrome b exists in fish neutrophils and the epitope is exposed to the cytoplasmic side of membrane, and suggest th at cytochrome b is conserved across species. (C) 1998 Elsevier Science Ltd. All rights reserved.