MUTUAL EDUCATION BETWEEN HEMATOPOIETIC-CELLS AND BONE-MARROW STROMAL CELLS THROUGH DIRECT CELL-TO-CELL CONTACT - FACTORS THAT DETERMINE THEGROWTH OF BONE-MARROW STROMA-DEPENDENT LEUKEMIC (HB-1) CELLS

A stroma-dependent cell line (HB-1) was established from myelogenous l eukemic cells of CBA/N mouse. Characterization of the cells showed tha t HB-1 proliferated on hematopoietic supportive stromal cells (MS-10), but did not survive or proliferate on hematopoietic nonsupportive cel ls (MS-K). Direct contact between HB-l and MS-IO appears to be necessa ry for HB-l to proliferate on MS-10. We found that interleukin-1 alpha (IL-1 alpha) produced by MS-10 plays a major role in the survival and proliferation of HB-1. IL-11 did not support the proliferation of HB- 1 cells by itself, but enhanced the proliferation of HB-1 cells in the presence of IL-1 alpha. The expression of IL-1 alpha and IL-11 was in duced in MS-10 by the direct contact with HB-1 cells, and the expressi on of IL-l receptor type I (IL-1RI) and interleukin-11 receptor (IL-11 R) was induced in HB-1 cells by the attachment of the cells Po MS-10. These findings show the existence of two-way interactions between HB-1 and MS-10. (C) 1998 by The American Society of Hematology.