EFFECTS OF THE NEUROPEPTIDE-Y ON ESTRADIOL AND PROGESTERONE SECRETIONBY HUMAN GRANULOSA-CELLS IN CULTURE

Objective: To investigate the possible effects of neuropeptide Y on st eroid release by human granulosa cells in culture. Design: Prospective study. Setting: A university laboratory and the division of obstetric s and gynecology in a hospital. Patient(s): Sixteen normally ovulating women. Intervention(s): Ovulation induction for IVF-ET with an LH-rel easing hormone analogue and gonodotropins. Main Outcome Measure(s): E- 2 and progesterone were assayed in the media conditioned by granulosa cells with the use of a double-antibody RIA. Result(s): Neuropeptide Y stimulates E-2 production in a dose-dependent fashion. Preincubation For 3 hours with hCG led to a statistically significant increase in ne uropeptide Y-induced E-2 secretion. In contrast, whereas 3 hours of pr eincubation with 10(-7) mol/L of neuropeptide Y did not elicit a stati stically significant increase in hCG-induced E-2 secretion, coincubati on for 48 hours significantly increased hCG-stimulated secretion. Unli ke E-2, progesterone secretion did not undergo any statistically signi ficant or dose-dependent variation after treatment with neuropeptide Y . Conclusion(s): Neuropeptide Y plays a role in human ovarian steroido genesis directly at the level of the granulosa cells of the follicles in the early stage of luteinization. In this way, neuropeptide Y could play an important role in controlling the positive feedback effect ex erted by the ovarian steroids on LH-releasing hormone and gonadotropin s in humans. (Fertil Steril(R) 1998;70:320-5. (C) 1998 by American Soc iety for Reproductive Medicine.).