A QUANTITATIVE CYTOCHROME-OXIDASE MAPPING STUDY, CROSS-REGIONAL AND NEUROBEHAVIORAL CORRELATIONS IN THE ANTERIOR FOREBRAIN OF AN ANIMAL-MODEL OF ATTENTION-DEFICIT HYPERACTIVITY DISORDER

The aim of this study was to trace by molecular imaging techniques the neural substrates of attention deficit hyperactivity disorder (ADHD) using the spontaneously hypertensive rat (SHR) as animal model. Adult SHR and Wistar-Kyoto (WKY) controls were used throughout this study. I n, experiment 1, naive male SHR and WKY were used, whereas in experime nt 2 SHR and WKY rats of both genders were trained on a multiple fixed interval (FI (120 s for water, 5-min extinction)) paradigm and sacrif iced 6 months later. In both experiments coronal sections of the anter ior forebrain were processed for quantitative cytochrome oxidase (COas e) histochemistry by the method of Gonzalez-Lima. Optical density valu es were transformed into actual enzyme activity units by using tissue- calibrated standards. In experiment 1, non-trained male rats of the SH R line showed lower COase activity in the medial and lateral prefronta l cortices, compared with WKY controls. In experiment 2, there was a l ine x treatment interaction effect in the pole of the nucleus accumben s (ACB). Regional correlative analyses revealed that: (i) under basal conditions, SHR are more synchronized than WKY rats in the COase level of different brain regions; and (ii) the training desynchronizes COas e activity in the WKY, further synchronizes it and increases the cross -talk between hemispheres in male SHR only. Neurobehavioral covariatio ns between behavioural scores and metabolic capacity in the medial and lateral prefrontal/frontal cortices, the caudate-putamen complex (CPU ), the pole, core, and shell of the accumbal complex (ACB), and the ve ntral pallidum (VP), indicated that, in the WKY rats, the frequency of lever pressing covaried positively with the COase activity in the CPU , whereas in the SHR covaried with both medial and lateral prefrontal/ frontal cortices. The bursts of activity during the 1-1.33-s segment w as positively correlated, in the WKY rats only, with the core and shel l of the ACB, and with the VP. Finally, the correlative profiles showe d significant gender differences with effects in male SHR only. Thus, the results lend support to the involvement of the cortico-striato-pal lidal system in ADHD. (C) 1998 Elsevier Science B.V. All rights reserv ed.