HPLC QUANTITATION OF MAJOR CAROTENOIDS OF FRESH AND PROCESSED GUAVA, MANGO AND PAPAYA

The major carotenoids in 52 samples of guava, mango papaya and some of their processed products were quantitated by HPLC, using both externa l standardization and standard addition calibration techniques. The re sults, submitted to the F and t tests, demonstrated that the precision and the means obtained by the two calibration procedures were nor sig nificantly different. The concentrations of beta-carotene and lycopene in guava, beta-carotene in mango and beta-carotene, beta-cryptoxanthi n and lycopene in papaya samples were coherent with those obtained pre viously with open column chromatography. The major problem in the HPLC quantitation of carotenoids is still obtaining and maintaining pure s tandards. (C) 1995 Academic Press Limited