ISOLATION AND MAPPING OF 2 PORCINE SKELETAL-MUSCLE MYOSIN HEAVY-CHAINISOFORMS

The present paper describes the isolation aad linkage mapping of two i soforms of skeletal muscle myosin heavy chain in pig. Two partial cDNA s (pAZMY4 and pAZMY7), coding for the porcine myosin heavy chain-2B an d -beta respectively, have been isolated from a pig skeletal muscle cD NA library. Four RFLPs were detected with the putative porcine skeleta l myosin heavy chain-2B probe (pAZMY4) and one RFLP was identified wit h the putative myosin heavy chain-beta probe (pAZMY7). Two myosin heav y chain loci were mapped by linkage analysis performed with the five R FLPs against the PiGMaP linkage consortium ResPig database: the MYH1 l ocus, which identifies the fast skeletal muscle myosin heavy chain gen e cluster, was located at the end of the map of porcine chromosome 12, while the MYH7 locus, which identifies the myosin heavy chain-alpha/- beta gene cluster, was assigned to the long arm of porcine chromosome 7.