CHARACTERIZATION OF FSH-REGULATED GENES ISOLATED BY MESSENGER-RNA DIFFERENTIAL DISPLAY FROM PIG OVARIAN GRANULOSA-CELLS

The present authors have isolated FSH-regulated genes from primary gra nulosa cell cultures with or without Follicle Stimulating Hormone (FSH ) treatment using mRNA differential display. mRNA differential display consists of amplification of partial sequences of cDNAs (150-400 bp) corresponding to 3' ends of cellular messenger RNAs, and thus, generat es 3' expressed sequence tags (3' ESTs). Five thousand cDNA bands were examined, among which the present authors have isolated and sequenced 16 different FSH-regulated products. These sequences were compared wi th those available in databases. Three of the sequences showed similar ity to identified genes from other species (bovine NADH dehydrogenase subunit 4, Xenopus chromosome sequence-associated polypeptide E and tr ansformation-sensitive protein IEF SSP) and four others with human EST s. Regulation of the corresponding genes has been checked by RT-PCR si nce most of these are expressed at a low level. FSH-regulation was con firmed for 12 mRNAs (four down- and eight up-regulated). The present a uthors have also mapped 12 of these ESTs on porcine chromosomes region s using a somatic cell hybrid panel.